Abstract

As control measures against malaria, my laboratory has focused on the interruption of sporogonic development of the parasite in the mosquito. The fact that most mosquitoes, other than a few Anopheline mosquitoes, are refractory to human malaria transmission suggests that blockades to Plasmodium development do exist in mosquitoes, and it will be useful to understand the nature of this blockade to reduce the transmission of malaria. The initial successes of transmission-blocking vaccines using antigens specific to mosquito stage of the parasite have demonstrated the potential of this approach. The major projects in this program can be classified under, the study of Plasmodium in mosquitoes. I. Mosquito midgut epithelium invasion by ookinete II. Ookinete adhesion to mosquito midgut epithelium III. Characterization of soluble mosquito factors that influence parasite development IV. Characterization of novel oocyst-specific molecules

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000811-01
Application #
6160802
Study Section
Special Emphasis Panel (LPD)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Shahabuddin, Mohammed (2002) Do Plasmodium ookinetes invade a specific cell type in the mosquito midgut? Trends Parasitol 18:157-61
Silva-Neto, Mario A C; Atella, Georgia C; Shahabuddin, Mohammed (2002) Inhibition of Ca2+/calmodulin-dependent protein kinase blocks morphological differentiation of plasmodium gallinaceum zygotes to ookinetes. J Biol Chem 277:14085-91
Wang, P; Conrad, J T; Shahabuddin, M (2001) Localization of midgut-specific protein antigens from Aedes aegypti (Diptera: Culicidae) using monoclonal antibodies. J Med Entomol 38:223-30
Shahabuddin, M; Costero, A (2001) Spatial distribution of factors that determine sporogonic development of malaria parasites in mosquitoes. Insect Biochem Mol Biol 31:231-40
Park, S S; Shahabuddin, M (2000) Structural organization of posterior midgut muscles in mosquitoes, Aedes aegypti and Anopheles gambiae. J Struct Biol 129:30-7
Killeen, G F; Foy, B D; Shahabuddin, M et al. (2000) Tagging bloodmeals with phagemids allows feeding of multiple-sample arrays to single cages of mosquitoes (Diptera: Culicidae) and the recovery of single recombinant antibody fragment genes from individual insects. J Med Entomol 37:528-33
Schneider, D; Shahabuddin, M (2000) Malaria parasite development in a Drosophila model. Science 288:2376-9
Zieler, H; Garon, C F; Fischer, E R et al. (2000) A tubular network associated with the brush-border surface of the Aedes aegypti midgut: implications for pathogen transmission by mosquitoes. J Exp Biol 203:1599-611
Zieler, H; Nawrocki, J P; Shahabuddin, M (1999) Plasmodium gallinaceum ookinetes adhere specifically to the midgut epithelium of Aedes aegypti by interaction with a carbohydrate ligand. J Exp Biol 202:485-95
Shahabuddin, M; Vinetz, J M (1999) Chitinases of human parasites and their implications as antiparasitic targets. EXS 87:223-34

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