Abstract

Cryptococcus neoformans is a pathogenic yeast which belongs to Basidiomycetes, a taxonomic Class phylogenetically remote from the rest of the pathogenic yeasts (Ascomycetes). C. neoformans causes fatal meningoencephalitis primarily in patients with T- lymphocyte dysfunction and is the only species in the genus Cryptococcus that has optimum growth at temperatures higher than 30C. Although extensive studies on the molecular basis of cell cycle and morphogenesis have been carried out in ascomycetous yeasts, such studies have not been conducted in C. neoformans. This project was initiated to study the molecular genetics of cell cycle associated genes in C. neoformans. In 2003, we reported the function of the gene CCN1 which was necessary for cell growth at 37C. During 2004-2005,we studied the effect of TUP1 deletion on the biology of MATa and MATalpha strains in serotype D. TUP1 is a global regulator, which is known to control morphogenesis in many ascomycetous fungi. Interestingly, tup1 mutants manifested quorum sensing (QS)and at growth temperatures at which QS functions differed between MATa and MATalpha strains. An 11 mer peptide secreted in the growth medium of tup1 mutant was identified as the quorum sensing molecule. Fungal QS factors reported thus far have been alcohols such as tyrosol or farnesol but a peptide has never been reported as a QS factor in Kingdom Fungi.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000847-07
Application #
7196653
Study Section
(LCI)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2005
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code