Abstract

Adenovirus and poxvirus vectors have shown promise for vaccines, but one key hurdle is preexisting immunity to these viruses in humans. The VRC has been investigating the use of DNA priming and alternative routes of immunization to overcome prior immunity. The results demonstrate that priming with a DNA expression vector before immunization with replication-defective adenovirus or poxvirus vectors can overcome the limitations imposed by preexisting immunity to the viral vectors. The VRC is also evaluating the route of delivery of the vaccine (mucosal delivery v. intramuscular) and its effects on the elicitation of cellular and humoral immunity in mice.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI005001-02
Application #
6822131
Study Section
(VIC)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2003
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Ko, Sung-Youl; Cheng, Cheng; Kong, Wing-Pui et al. (2009) Enhanced induction of intestinal cellular immunity by oral priming with enteric adenovirus 41 vectors. J Virol 83:748-56
Wang, Lingshu; Cheng, Cheng; Ko, Sung-Youl et al. (2009) Delivery of human immunodeficiency virus vaccine vectors to the intestine induces enhanced mucosal cellular immunity. J Virol 83:7166-75
Cheng, Cheng; Gall, Jason G D; Kong, Wing-pui et al. (2007) Mechanism of ad5 vaccine immunity and toxicity: fiber shaft targeting of dendritic cells. PLoS Pathog 3:e25
Lemiale, Franck; Haddada, Hedi; Nabel, Gary J et al. (2007) Novel adenovirus vaccine vectors based on the enteric-tropic serotype 41. Vaccine 25:2074-84
Letvin, Norman L; Mascola, John R; Sun, Yue et al. (2006) Preserved CD4+ central memory T cells and survival in vaccinated SIV-challenged monkeys. Science 312:1530-3
Mattapallil, Joseph J; Douek, Daniel C; Buckler-White, Alicia et al. (2006) Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge. J Exp Med 203:1533-41
Acierno, Paula M; Schmitz, Jorn E; Gorgone, Darci A et al. (2006) Preservation of functional virus-specific memory CD8+ T lymphocytes in vaccinated, simian human immunodeficiency virus-infected rhesus monkeys. J Immunol 176:5338-45
Wu, Lan; Kong, Wing-Pui; Nabel, Gary J (2005) Enhanced breadth of CD4 T-cell immunity by DNA prime and adenovirus boost immunization to human immunodeficiency virus Env and Gag immunogens. J Virol 79:8024-31
Mascola, John R; Sambor, Anna; Beaudry, Kristin et al. (2005) Neutralizing antibodies elicited by immunization of monkeys with DNA plasmids and recombinant adenoviral vectors expressing human immunodeficiency virus type 1 proteins. J Virol 79:771-9
Santra, Sampa; Seaman, Michael S; Xu, Ling et al. (2005) Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates. J Virol 79:6516-22

Showing the most recent 10 out of 13 publications