Abstract

In this study, we have produced soluble versions of Env glycoproteins derived from a primary isolate stabilized by heterologous trimerization motifs (foldon or GDN4). We have tested these glycoproteins for immunogenicity in rabbits and guinea pigs. Breadth of neutralization indicate that these glycoproteins are interesting platforms for furthere modification to better elicit broadly neutralizing antibodies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI005069-01
Application #
7006207
Study Section
(VRC)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2004
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Elopre, Latesha; Kudroff, Kachina; Westfall, Andrew O et al. (2017) Brief Report: The Right People, Right Places, and Right Practices: Disparities in PrEP Access Among African American Men, Women, and MSM in the Deep South. J Acquir Immune Defic Syndr 74:56-59
Dosenovic, Pia; Chakrabarti, Bimal; Soldemo, Martina et al. (2009) Selective expansion of HIV-1 envelope glycoprotein-specific B cell subsets recognizing distinct structural elements following immunization. J Immunol 183:3373-82
Morner, Andreas; Douagi, Iyadh; Forsell, Mattias N E et al. (2009) Human immunodeficiency virus type 1 env trimer immunization of macaques and impact of priming with viral vector or stabilized core protein. J Virol 83:540-51
Doria-Rose, Nicole A; Klein, Rachel M; Manion, Maura M et al. (2009) Frequency and phenotype of human immunodeficiency virus envelope-specific B cells from patients with broadly cross-neutralizing antibodies. J Virol 83:188-99
Scheid, Johannes F; Mouquet, Hugo; Feldhahn, Niklas et al. (2009) Broad diversity of neutralizing antibodies isolated from memory B cells in HIV-infected individuals. Nature 458:636-40
Li, Y; Svehla, K; Mathy, N L et al. (2006) Characterization of antibody responses elicited by human immunodeficiency virus type 1 primary isolate trimeric and monomeric envelope glycoproteins in selected adjuvants. J Virol 80:1414-26
Bower, Joseph F; Li, Yuxing; Wyatt, Richard et al. (2006) HIV-1 Envgp140 trimers elicit neutralizing antibodies without efficient induction of conformational antibodies. Vaccine 24:5442-51
Pancera, Marie; Lebowitz, Jacob; Schon, Arne et al. (2005) Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis. J Virol 79:9954-69
Grundner, Christoph; Li, Yuxing; Louder, Mark et al. (2005) Analysis of the neutralizing antibody response elicited in rabbits by repeated inoculation with trimeric HIV-1 envelope glycoproteins. Virology 331:33-46
Forsell, Mattias N E; Li, Yuxing; Sundback, Maria et al. (2005) Biochemical and immunogenic characterization of soluble human immunodeficiency virus type 1 envelope glycoprotein trimers expressed by semliki forest virus. J Virol 79:10902-14

Showing the most recent 10 out of 12 publications