We continued research on the molecular mechanism of meiotic recombination in the yeast Saccharomyces cerevisiae, with a focus on three aspects of this complex biological process. We examined the initiation event in meiotic recombination, double-strand DNA break formation, with the aim of identifying the enzyme(s) responsible for break formation, their substrate requirements, and the factors that control the location and frequency of breaks. These factors include aspects of both chromatin and higher-order chromosome structure. A particular focus has been the role of chromosome structural elements, specifically centromeres and telomeres, in repressing double-strand break formation. We are also examining the relationship between meiotic DNA replication and recombination, and have demonstrated a direct mechanistic link between these two processes. We developed techniques to isolate unstable intermediates in meiotic recombination and are currently analyzing their structure. We also studied the relationship between recombination and chromosome pairing and recombination events that occur during meiosis. - Chromatin, chromosome structure, DNA repair, Meiosis, recombination, Saccharomyces cerevisiae, yeast, DNA replication, - Neither Human Subjects nor Human Tissues

National Institute of Health (NIH)
National Cancer Institute (NCI)
Intramural Research (Z01)
Project #
Application #
Study Section
Special Emphasis Panel (LB)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
National Cancer Institute Division of Basic Sciences
United States
Zip Code
Nakamura, Asako; Sedelnikova, Olga A; Redon, Christophe et al. (2006) Techniques for gamma-H2AX detection. Methods Enzymol 409:236-50
Lichten, Michael (2005) Rad50 connects by hook or by crook. Nat Struct Mol Biol 12:392-3
Jessop, Lea; Allers, Thorsten; Lichten, Michael (2005) Infrequent co-conversion of markers flanking a meiotic recombination initiation site in Saccharomyces cerevisiae. Genetics 169:1353-67
Borde, Valerie; Lin, Waka; Novikov, Eugene et al. (2004) Association of Mre11p with double-strand break sites during yeast meiosis. Mol Cell 13:389-401
Schlecht, Helene B; Lichten, Michael; Goldman, Alastair S H (2004) Compartmentalization of the yeast meiotic nucleus revealed by analysis of ectopic recombination. Genetics 168:1189-203
Shroff, Robert; Arbel-Eden, Ayelet; Pilch, Duane et al. (2004) Distribution and dynamics of chromatin modification induced by a defined DNA double-strand break. Curr Biol 14:1703-11
Unal, Elcin; Arbel-Eden, Ayelet; Sattler, Ulrike et al. (2004) DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain. Mol Cell 16:991-1002
Borde, V; Goldman, A S; Lichten, M (2000) Direct coupling between meiotic DNA replication and recombination initiation. Science 290:806-9
Allers, T; Lichten, M (2000) A method for preparing genomic DNA that restrains branch migration of Holliday junctions. Nucleic Acids Res 28:e6
Gerton, J L; DeRisi, J; Shroff, R et al. (2000) Inaugural article: global mapping of meiotic recombination hotspots and coldspots in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 97:11383-90

Showing the most recent 10 out of 14 publications