Our research objective is to understand the molecular and genetic mechanisms responsible for cell growth, differentiation and neoplastic transformation. We study the oncogenes, tumor-suppressor genes and signal-transducing proteins involved in BALB/c mouse plasmacytomas, B-cell lymphomas and other mouse and human experimental tumor systems. These are valuable experimental models, because they have many biological and molecular genetic features in common with human multiple myeloma, non-Hodgkin's lymphomas, and other human malignancies that are in need of mechanistic understanding in order to devise more specific therapy and preventive measures. BALB/c plasmacytomas, like rat immunocytomas and human Burkitt lymphomas, are characterized by constitutive expression of messenger RNA and protein from the master oncogene, c-Myc. Most commonly, c-Myc expression in plasmacytomas is dysregulated secondary to a chromosomal translocation in the vicinity of the c-Myc gene. It is still not clear why dysregulated expression of the c-Myc oncogene leads to many different forms of tumors in human and mouse cells or which additional mutations are required for complete transformation. One mechanism that may be important consequence of altered Myc expression is genomic instability, in the form of extra- and intra-chromosomal amplification, and the subsequent overexpression of several genes that are crucial for DNA synthesis and cell proliferation, including cyclin D2. Others, such as ribonucleotide reductase subunit 2 (RNR2) are amplified but not overexpressed. We are actively engaged in learning how many such genes can be amplified by this mechanism and what determines their expression or lack thereof. Another important finding from this study is the discovery that cyclin D2 is a direct target of expression activation by the proto-oncogene, c-Myc. In the study of signal transduction, we are investigating protein kinase C (PKC), a multigene family of at least 12 structurally related isoenzymes that are important mediators of many forms of signal transduction. Using a variety of expression vectors, we have overexpressed many of the PKCs in fibroblasts, lymphocytic and myeloid cell lines. This has made possible the identification of specific functions and intracellular targets for the individual PKC isoenzymes. We have been focusing on the delta and epsilon isoenzymes, which seem to have opposite effects on cell proliferation. We have shown that PKC-delta is responsible for myeloid differentiation and growth inhibition, while overexpressed PKC-epsilon stimulates cell growth and transforms fibroblasts into tumor cells. We are dissecting the structure of these isoenzymes to determine which protein domains control these functions. We have shown that most of the isoenzyme-specific determinants are located in the catalytic half (the carboxyl-terminal domain) of these PKCs by creating reciprocal chimeric cDNAs that encode molecules that are half PKC-delta and half PKC-epsilon. Chimeric molecules that have carboxyl-terminal PKC-delta sequences are able to cause macrophage differentiation much like the parent all-PKC-delta protein. Similarly, a PKC chimera with a PKC-epsilon carboxyl-terminus, retains the neoplastic transformation potential of the all-PKC-epsilon protein. We are further dissecting the structure of the catalytic domain to determine which sub-domains determine PKC isoform-specific functions. We are also studying the nature of PKC's involvement in plasmacytoma induction and apoptosis, in cytoskeleton-related changes in cell shape, and in metastasis of these and other types of tumors, including human prostate cancer. We have shown that phorbol ester-activation of overexpressed PKC-delta disrupts the actin cytoskeleton in human and mouse lymphocytes, leading to the loss of membrane ruffling, a surface alteration needed for cell movement, and the loss of the typical elongated shape of these cells. We think that this effect is due to PKC-mediated changes in the phosphorylation of the adaptor molecule, paxillin. We have embarked on a series of studies of alterations in gene expression during signal transduction and neoplastic transformation using differential display and using microarray hybridization. Collaborators on this research include Charles Vinson, Ph.D., Peter Blumberg, Ph.D., Qiming Wang, Ph.D. & Jane Trepel, NCI; Sabine Mai, Ph.D., & Theodore Kuschak, Ph.D. Univ. of Manitoba, Winnipeg, Canada, Larisa Romanova, M.D., Ph.D., Harvard Medical School and Harald Mischak, Ph.D., & Michael Kracht, Medizinische Hochschule Hannover, Hannover, Germany.

Agency
National Institute of Health (NIH)
Institute
Division of Basic Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC008727-23
Application #
6559013
Study Section
(LG)
Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Park, Eun Sung; Lee, Ju-Seog; Woo, Hyun Goo et al. (2007) Heterologous tissue culture expression signature predicts human breast cancer prognosis. PLoS One 2:e145
Wang, Hongyang; Owens, James D; Shih, Joanna H et al. (2006) Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics 7:97
Thiefes, Axel; Wolter, Sabine; Mushinski, J Frederic et al. (2005) Simultaneous blockade of NFkappaB, JNK, and p38 MAPK by a kinase-inactive mutant of the protein kinase TAK1 sensitizes cells to apoptosis and affects a distinct spectrum of tumor necrosis factor [corrected] target genes. J Biol Chem 280:27728-41
Park, Sung Sup; Kim, Joong Su; Tessarollo, Lino et al. (2005) Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice. Cancer Res 65:1306-15
Romanova, Larisa Y; Hashimoto, Shigeru; Chay, Kee-Oh et al. (2004) Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J Cell Sci 117:3759-68
Wang, Qiming Jane; Lu, Ganwei; Schlapkohl, Walter A et al. (2004) The V5 domain of protein kinase C plays a critical role in determining the isoform-specific localization, translocation, and biological function of protein kinase C-delta and -epsilon. Mol Cancer Res 2:129-40
Mai, Sabine; Mushinski, J Frederic (2003) c-Myc-induced genomic instability. J Environ Pathol Toxicol Oncol 22:179-99
Kim, J S; Pirnia, F; Choi, Y H et al. (2000) Lovastatin induces apoptosis in a primitive neuroectodermal tumor cell line in association with RB down-regulation and loss of the G1 checkpoint. Oncogene 19:6082-90
Kuschak, T I; Taylor, C; McMillan-Ward, E et al. (1999) The ribonucleotide reductase R2 gene is a non-transcribed target of c-Myc-induced genomic instability. Gene 238:351-65
Romanova, L Y; Alexandrov, I A; Blagosklonny, M V et al. (1999) Regulation of actin cytoskeleton in lymphocytes: PKC-delta disrupts IL-3-induced membrane ruffles downstream of Rac1. J Cell Physiol 179:157-69

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