The success of antiviral therapies will require a detailed knowledge of proteins involved in the replication, assembly and infection of HIV-1 and HIV-2. Central to these processes is the enzyme reverse transcriptase (RT), which has been a leading target for antiviral drugs for over a decade. Since RT-mediated replication of the HIV RNA genome is a multi-step process, dissecting and understanding each event at the molecular level will have the consequence of expanding the repertoire of therapeutic targets. Moreover, current evidence suggests RT may not work alone in replicating the HIV genome, but rather may recruit additional viral proteins. A better understanding of the 'communication' between these accessory proteins and RT can therefore offer additional therapeutic avenues. Research in the Reverse Transcriptase Biochemistry Laboratory exploits recombinant DNA technology to accurately recapitulate steps in HIV replication and evaluate their sensitivity to antiviral agents, using RTs of HIV-1, HIV-2 and their counterparts from closely-related viruses. Furthermore, since alterations in RT structure result in the rapid acquisition of drug resistance and failure of antiviral therapies, it is equally important to understand the exact nature of these subtle alterations. Thus, in addition to a biochemical analysis of HIV RT, the laboratory collaborates with additional groups at the National Cancer Institute to obtain high resolution structural information on both wild type and mutant variants of this highly versatile enzyme.
| Rausch, Jason W; Lener, Daniela; Miller, Jennifer T et al. (2002) Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity. Biochemistry 41:4856-65 |
| Rausch, J W; Sathyanarayana, B K; Bona, M K et al. (2000) Probing contacts between the ribonuclease H domain of HIV-1 reverse transcriptase and nucleic acid by site-specific photocross-linking. J Biol Chem 275:16015-22 |
| Powell, M D; Beard, W A; Bebenek, K et al. (1999) Residues in the alphaH and alphaI helices of the HIV-1 reverse transcriptase thumb subdomain required for the specificity of RNase H-catalyzed removal of the polypurine tract primer. J Biol Chem 274:19885-93 |
