Abstract

The goals of this project are to determine the frequency and mechanisms of HIV-1 recombination and to examine recombination between divergent lentiviruses. We have recently measured HIV-1 recombination rates and found that two markers 1.0 kb apart recombine at a rate approaching the 50% maximum measurable rate. Therefore, recombination is a very powerful tool for reassorting the HIV-1 genome; markers separated by a short distance are apparently unlinked during the process. We are studying HIV-1 recombination further by examining the relationship between recombination rates and marker distances shorter than 1 kb, by characterizing the locations and frequencies of recombination events in the HIV-1 genome, and by investigating whether high negative interference exists in HIV-1 recombination. We are also attempting to decipher the major mechanisms of HIV-1 recombination.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Basic Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC010504-02
Application #
7058970
Study Section
(RML)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2004
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Moore, Michael D; Chin, Mario P S; Hu, Wei-Shau (2009) HIV-1 recombination: an experimental assay and a phylogenetic approach. Methods Mol Biol 485:87-105
Nikolaitchik, Olga A; Gorelick, Robert J; Leavitt, Maria G et al. (2008) Functional complementation of nucleocapsid and late domain PTAP mutants of human immunodeficiency virus type 1 during replication. Virology 375:539-49
Chin, Mario P S; Chen, Jianbo; Nikolaitchik, Olga A et al. (2007) Molecular determinants of HIV-1 intersubtype recombination potential. Virology 363:437-46
Fu, William; Prasad, V V S P; Chen, Jianbo et al. (2007) Molecular mechanisms of simian immunodeficiency virus SIV(agm) RNA encapsidation. Virology 363:210-9
Nikolaitchik, Olga; Rhodes, Terence D; Ott, David et al. (2006) Effects of mutations in the human immunodeficiency virus type 1 Gag gene on RNA packaging and recombination. J Virol 80:4691-7
Chen, Jianbo; Powell, Douglas; Hu, Wei-Shau (2006) High frequency of genetic recombination is a common feature of primate lentivirus replication. J Virol 80:9651-8
Chin, Mario P S; Rhodes, Terence D; Chen, Jianbo et al. (2005) Identification of a major restriction in HIV-1 intersubtype recombination. Proc Natl Acad Sci U S A 102:9002-7
Chen, Jianbo; Rhodes, Terence D; Hu, Wei-Shau (2005) Comparison of the genetic recombination rates of human immunodeficiency virus type 1 in macrophages and T cells. J Virol 79:9337-40
Rhodes, Terence D; Nikolaitchik, Olga; Chen, Jianbo et al. (2005) Genetic recombination of human immunodeficiency virus type 1 in one round of viral replication: effects of genetic distance, target cells, accessory genes, and lack of high negative interference in crossover events. J Virol 79:1666-77
Svarovskaia, Evguenia S; Cheslock, Sara R; Zhang, Wen-Hui et al. (2003) Retroviral mutation rates and reverse transcriptase fidelity. Front Biosci 8:d117-34

Showing the most recent 10 out of 12 publications