Abstract

We have used reverse phase protein microarray (RPMA) to assess tumor and host-organ proteomic signaling responses to high-dose chemotherapy delivered during isolated hepatic perfusion (IHP) surgery and continuous hyperthermic peritoneal perfusion surgery for patients with metastatic disease confined to liver and advanced abdominal cancers. RPMA allows for real-time protein network interrogation in high-throughput and parallel fashion through the use of robotic arrayers, isoform specific antibodies, and mathematical modeling. The Laser Capture Microdissection (LCM) Core and the Proteomic Core provide support for a diverse spectrum of investigators and clinical protocols. In 2006, samples were accrued and or analyzed from 12 NCI clinical protocols using reverse phase protein microarrays. More than 50 investigators were trained in laser capture technology. LCM support was provided for 34 experimental protocols involving investigators from the NCI, as well as NIDCR, NIA, NHLBI, NEI, NHGRI, NIAID, NIMH, and NICHD.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Basic Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC010676-02
Application #
7338781
Study Section
(LP)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2006
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Davidson, Ben; Espina, Virginia; Steinberg, Seth M et al. (2006) Proteomic analysis of malignant ovarian cancer effusions as a tool for biologic and prognostic profiling. Clin Cancer Res 12:791-9
Azad, Nilofer S; Rasool, Nabila; Annunziata, Christina M et al. (2006) Proteomics in clinical trials and practice: present uses and future promise. Mol Cell Proteomics 5:1819-29
Quintanilla-Martinez, Leticia; Pittaluga, Stefania; Miething, Cornelius et al. (2006) NPM-ALK-dependent expression of the transcription factor CCAAT/enhancer binding protein beta in ALK-positive anaplastic large cell lymphoma. Blood 108:2029-36
Kobrin, Carol; Cha, Soung-Chul; Qin, Hong et al. (2006) Molecular analysis of light-chain switch and acute lymphoblastic leukemia transformation in two follicular lymphomas: implications for lymphomagenesis. Leuk Lymphoma 47:1523-34
Campbell, Michael J; Esserman, Laura J; Zhou, Yamei et al. (2006) Breast cancer growth prevention by statins. Cancer Res 66:8707-14
Srinivasan, Ramaprasad; Daniels, Jasmine; Fusaro, Vincent et al. (2006) Accurate diagnosis of acute graft-versus-host disease using serum proteomic pattern analysis. Exp Hematol 34:796-801
Rudelius, Martina; Pittaluga, Stefania; Nishizuka, Satoshi et al. (2006) Constitutive activation of Akt contributes to the pathogenesis and survival of mantle cell lymphoma. Blood 108:1668-76
Petricoin 3rd, Emanuel F; Bichsel, Verena E; Calvert, Valerie S et al. (2005) Mapping molecular networks using proteomics: a vision for patient-tailored combination therapy. J Clin Oncol 23:3614-21
Gulmann, Christian; Espina, Virginia; Petricoin 3rd, Emanuel et al. (2005) Proteomic analysis of apoptotic pathways reveals prognostic factors in follicular lymphoma. Clin Cancer Res 11:5847-55
Feldman, Andrew L; Berthold, Frank; Arceci, Robert J et al. (2005) Clonal relationship between precursor T-lymphoblastic leukaemia/lymphoma and Langerhans-cell histiocytosis. Lancet Oncol 6:435-7

Showing the most recent 10 out of 16 publications