Analysis of macromolecular enzymatic processes is based largely on indirect observations. We developed a direct imaging approach in which one or more macromolecular components are labeled with Quantum Dots (Q-dots), allowing one to follow the behavior of a population of individual molecules in real-time. Q-dots nano-biochemistry provides an unprecedented simple and direct analysis of macromolecular complexes in action. This system can be adapted for proteomics and drug discovery. We have chosen to pioneer the monitoring of single macromolecular events by the attachment of Q-dots to DNA, RNA and proteins without interfering with their biological activity. This method provides a missing link between analysis of complex interactions in transcription complexes in vivo (ChIP analysis) and results obtained by traditional bulk biochemistry. This system can also be adapted for proteomics and drug discovery.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC010758-03
Application #
7733198
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2008
Total Cost
$95,014
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code