Pure populations of primary lymphocytes, monocytes, and dendritic cells will be separated from normal human blood via physical cell separation and FACS. Monoclonal antibody (MAb) markers defining various differentiated cell phenotypes will be used to identify and purify specific subpopulations of these cell types. The cells will be infected with HIV-1 and virus growth and gene expression will be evaluated by FACS, PCR, and other assays. Cell subpopulations which support HIV replication in vitro can then be assessed from HIV-seropositive blood to determine which subpopulations are viral reservoirs in vivo.

Agency
National Institute of Health (NIH)
Institute
Food and Drug Administration (FDA)
Type
Intramural Research (Z01)
Project #
1Z01BG006045-01
Application #
3804857
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Center Biologics Evaluation Research Transfusion
Department
Type
DUNS #
City
State
Country
United States
Zip Code