We have characterized several new strains of the various HIV subtypes ranging from A-G of group M, isolates of group O and HIV-2. We have also obtained several drug resistant strains of HIV. We are sequencing the regulatory genes tat, nef, vpu and vpr to look for any possible relationships between these sequence s and co-receptor usage. Studies are underway to evaluate the replication of non clade B, O viruses and drug resistant viruses in in vitro cell systems with specific emphasis on their cell trpoism, replication kinetics, chemokine and cytokine usage. We are in the final stages of developing strategies in Cameroon (Africa) to identify new variants and recombinants of HIV and related viruses. These viruses will be investigated for their infectivity, drug susceptibility and pathogenesis in appropriate cell culture systems and animal models. We have examined the impact of HIV variation on apoptosis, chemokine and cytokine production in donor PBLs. We will also examine the role of tat and nef in faciltitating fasL mediated apoptosis in PBLs and dendritic cells. These studies will be also carried out in SCID mice. Effects of tat and nef on the regulation of chemokines in susceptible dendritic cells are being investigated. The infectivity of different HIV subtypes and variants for dendritic cells and macrophages are being studied.

Agency
National Institute of Health (NIH)
Institute
Bureau of Health Planning and Resources Development (CBERTTD)
Type
Intramural Research (Z01)
Project #
1Z01BP002001-09
Application #
6545922
Study Section
(LMV)
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Health Planning & Resources Development
Department
Type
DUNS #
City
State
Country
United States
Zip Code