Neutral glycolipids are differentially expressed in functionally distinct subpopulations of murine lymphocytes. Subpopulations of B cells can be studied by examining hyrbidoma lines derived from fusion of splenic B lymphocytes with the mouse myeloma SP2/0. We are analyzing total neutral glycolipids from hybridomas by thin layer chromatography and by GC/MS analysis of oligosaccharides after trifluoroacetolysis. Hybridomas from Balb/c splenocytes express glycolipids containing from two to five simple sugars. These include globoside and its precursors as well as asialo-GM2 and 2' fucosullactosyl ceramide. The goal os this project is to correlate expression of olgiosaccharide chains of glycolipids with functional parameters of B cell subsets such as responsiveness to Type I and Type II antigens.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Biology And Diagnosis (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB000556-03
Application #
4691699
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code