Studies on the structural and functional organization of type II intracisternal A-particle retrovirus elements at the molecular level have continued. We have shown that methylation and association with two defined repetitive sequences appear to play a role in controlling expression of these genes in mouse cells. On the bases on sequence, a peptide representing the viral integrase has been synthesized and used to raise antibodies in rabbits. The antibody will permit us to study the integrase function in a myeloma cell line in which extensive amplification of these IAP sequences has occurred. A cDNA library has been made from this cell line. Clones for further study have been selected on the basis of IAP protein expression as well a sequence homology. Cloning of the integrase gene will allow characterization of this protein.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Biology And Diagnosis (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CB005264-07
Application #
3939267
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Cancer Biology and Diagnosis
Department
Type
DUNS #
City
State
Country
United States
Zip Code