A competitive inhibition radioimmunoassay has been developed for quantitative analysis of the HBsAg-binding polymerized human albumin. Based on this assay, we have shown that specific binding for glutaraldehyde polymerized albumin is at least 1,000 fold stronger than human albumin polymerized by other procedures such as heat, aging and carbodiimide cross-linking. The specific binding site for polymerized albumin is localized on the pre-S gene product of HBsAg. An assay procedure for antibody activity to polymerized albumin has been developed.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL002043-05
Application #
3916482
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code