Peptidyl leukotrienes signal through at least two cysteinyl leukotriene receptors. The control of expression of these two receptors is not well understood. The current project will propose to study the effect of proinflammatory cytokines on gene and protein expression of both receptors in human cells. The structure of the two genes and of the 5' promoter region of each has been determined. Assays of gene expression, steady state mRNA expression, and cellular protein expression have been developed. Human cell lines which express either LTR1 or LTR2 are being studied. A manuscript is published, and a second manuscript in in review.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL008071-05
Application #
7733594
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2008
Total Cost
$228,794
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Woszczek, Grzegorz; Chen, Li-Yuan; Nagineni, Sahrudaya et al. (2007) IFN-gamma induces cysteinyl leukotriene receptor 2 expression and enhances the responsiveness of human endothelial cells to cysteinyl leukotrienes. J Immunol 178:5262-70
Woszczek, Grzegorz; Pawliczak, Rafal; Qi, Hai-Yan et al. (2005) Functional characterization of human cysteinyl leukotriene 1 receptor gene structure. J Immunol 175:5152-9