This study will compare 3 monoclonal Pneumocystis carinii immunofluorescence test kits for sensitivity and specificity in detection of Pneumocystis carinii in respiratory specimens. The three kits are: 1) Monoclonal antibody (MAB) Indirect Fluorescent Antibody (NIH); 2) MAB Direct Fluorescent Antibody (Genetic Systems Corporation, Seattle, Washington, 98121), and 3) MAB Indirect Fluorescent Antibody; Fluoroslide Pneumocystis carinii (Disease Detection International, IME, Irvine, California). Induced sputums and bronchial lavages from AIDS patients as well as non-AIDS patients will be tested for the presence of Pneumocystis carinii. The patient population will include pediatric as well as adult patients to determine sensitivity and specificity of the different commercial kits to see which one is best to use for detecting Pneumocystis carinii in respiratory specimens.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL010185-02
Application #
3853123
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code