This ongoing project involves several stages. We have a protocol for collecting of bone marrow from normal volunteers to use for the culture of relatively pure populations of megakaryocytes. We were in the process of developing the culture conditions for megakaryocyte enrichment, but commercial culture kits recently became available for this purpose. Over the past year, we have been developing an assay for detecting mutations in somatic DNA using representational difference analysis. When this assay works reliably with test samples, we will begin to analyze patient specimens.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL010238-03
Application #
2571431
Study Section
Cognition and Perception Study Section (CP)
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code