Use of Pcr and Rflp Analysis for Identification of Mycobacteria and Nocardia

Witebsky, F G.

Abstract

PCR amplification of a portion of the genome of both rapidly-growing mycobacteria and nocardia, followed by RFLP analysis of the amplification products, will be evaluated to assess the utility of these procedures for use in the diagnostic laboratory. Particular attention will be paid to the speed and sensitivity for identification purposes of these procedures as compared with conventional identification techniques. Cost, technologist time, and level of expertise required will also be assessed.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Clinical Center (CLC)
• Type: Intramural Research (Z01)
• Project #: 1Z01CL010247-01
• Application #: 5201202
• Support Year: 1
• Fiscal Year: 1995

Institution

• Name: Clinical Center
• Country: United States

Publications

Palmore, Tara N; Shea, Yvonne R; Conville, Patricia S et al. (2009) ""Mycobacterium tilburgii,"" a newly described, uncultivated opportunistic pathogen. J Clin Microbiol 47:1585-7

Montero, C I; Shea, Y R; Jones, P A et al. (2008) Evaluation of Pyrosequencing technology for the identification of clinically relevant non-dematiaceous yeasts and related species. Eur J Clin Microbiol Infect Dis 27:821-30

Conville, Patricia S; Brown, June M; Steigerwalt, Arnold G et al. (2008) Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the ""Nocardia transvalensis Complex"". J Clin Microbiol 46:1178-84

Conville, Patricia S; Witebsky, Frank G (2007) Organisms designated as Nocardia asteroides drug pattern type VI are members of the species Nocardia cyriacigeorgica. J Clin Microbiol 45:2257-9

Conville, Patricia S; Witebsky, Frank G (2007) Analysis of multiple differing copies of the 16S rRNA gene in five clinical isolates and three type strains of Nocardia species and implications for species assignment. J Clin Microbiol 45:1146-51

Conville, Patricia S; Zelazny, Adrian M; Witebsky, Frank G (2006) Analysis of secA1 gene sequences for identification of Nocardia species. J Clin Microbiol 44:2760-6

Conville, Patricia S; Witebsky, Frank G (2005) Multiple copies of the 16S rRNA gene in Nocardia nova isolates and implications for sequence-based identification procedures. J Clin Microbiol 43:2881-5

Cloud, Joann L; Conville, Patricia S; Croft, Ann et al. (2004) Evaluation of partial 16S ribosomal DNA sequencing for identification of nocardia species by using the MicroSeq 500 system with an expanded database. J Clin Microbiol 42:578-84

Conville, Patricia S; Brown, June M; Steigerwalt, Arnold G et al. (2004) Nocardia kruczakiae sp. nov., a pathogen in immunocompromised patients and a member of the ""N. nova complex"". J Clin Microbiol 42:5139-45

Conville, Patricia S; Brown, June M; Steigerwalt, Arnold G et al. (2003) Nocardia veterana as a pathogen in North American patients. J Clin Microbiol 41:2560-8