Goals of this project are to improve clinical laboratory methods for diagnosis of disease. Studies include analysis of clinical laboratory practices, analysis of the accuracy of laboratory tests, and development of new tests and testing technologies. The major efforts over the past year have been to continue work on the effect of blood collection tubes on assays, to characterize urinary proteins of potential diagnostic significance, and to evaluate the distribution of homocysteine between different plasma protein fractions. 1) Analyses of effects of blood collection tubes found complex interactions with the analysis of free fatty acids probably due to modification of the specimen rather than direct interaction with the assay. These efforts help identify optimal collection procedures and tubes for collection of specimens for free fatty acid analysis and point to new mechanisms by which laboratory results may be affected by collection tubes. 2) Studies of urinary proteins have focused mainly on analysis of urinary albumin which serves as one of the most widely used markers for early glomerular injury. Our laboratory has examined how clinical assays measure modified forms of albumin that may occur in urine and have undertaken efforts to characterize the modified forms of albumin in urine. These efforts are expected to contribute to better clinical application and interpretation of urinary albumin measurements for the detection of kidney injury. 3) Plasma homocysteine has been of great interest as a potential risk factor for cardiovascular disease and thrombosis. We have examined the linkage of homocysteine to plasma proteins via disulfide bonds. Our analyses may subdivide the total homocysteine content into different subfractions bound to different proteins, and we have examined the differential exchange of homocysteine bound to different protein fractions. These studies seek to understand the interactions of homocysteine with plasma proteins and to identify subfractions of homocysteine that may serve as better indicators of cardiovascular disease.

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Intramural Research (Z01)
Project #
1Z01CL010342-05
Application #
7593113
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2007
Total Cost
$55,000
Indirect Cost
Name
Clinical Center
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Notarangelo, Luigi D; Fleisher, Thomas A (2017) Targeted strategies directed at the molecular defect: Toward precision medicine for select primary immunodeficiency disorders. J Allergy Clin Immunol 139:715-723
Sviridov, Denis; Owen, William E; Roberts, William L et al. (2009) Proteinuria without albuminuria: urinary protein excretion by a subset of patients with burn injuries. Clin Chim Acta 403:42-46
Lane, Jason W; Rehak, Nadja N; Hortin, Glen L et al. (2008) Pseudohyperphosphatemia associated with high-dose liposomal amphotericin B therapy. Clin Chim Acta 387:145-9
Sviridov, Denis; Drake, Steven K; Hortin, Glen L (2008) Reactivity of urinary albumin (microalbumin) assays with fragmented or modified albumin. Clin Chem 54:61-8
Hortin, Glen L (2008) Of immunounreactive urinary albumin and unicorns. Am J Clin Pathol 130:314-5
Hortin, Glen L; Sviridov, Denis; Anderson, N Leigh (2008) High-abundance polypeptides of the human plasma proteome comprising the top 4 logs of polypeptide abundance. Clin Chem 54:1608-16
Rehak, Nadja N; Cecco, Stacey A; Hortin, Glen L (2008) Photolysis of bilirubin in serum specimens exposed to room lighting. Clin Chim Acta 387:181-3
Hortin, Glen L (2007) A new era in protein quantification in clinical laboratories: application of liquid chromatography-tandem mass spectrometry. Clin Chem 53:543-4
Bowen, Raffick A R; Vu, Chi; Remaley, Alan T et al. (2007) Differential effect of blood collection tubes on total free fatty acids (FFA) and total triiodothyronine (TT3) concentration: a model for studying interference from tube constituents. Clin Chim Acta 378:181-93
Seam, Nitin; Gonzales, Denise A; Kern, Steven J et al. (2007) Quality control of serum albumin depletion for proteomic analysis. Clin Chem 53:1915-20

Showing the most recent 10 out of 34 publications