Genetic mutagenesis and biochemical approaches have been employed to study the role of ras oncogenes in cell transformation and ras proto-oncogenes in normal cell function. By site-directed mutagenesis of the H-ras oncogene at the NKXD consensus motif of the GTP-binding site, we have identified several mutants derived from the viral H-ras oncogene by substituting the asparagine-116 residue with tyrosine and isoleucine that are trans-dominant suppressors of ras activities. Studies are in progress to delineate ras functions employing these dominant negative mutants of ras oncogenes.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CP004963-14
Application #
3874614
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Division of Cancer Epidemiology and Genetics
Department
Type
DUNS #
City
State
Country
United States
Zip Code