In order to elucidate the structure and function of the human cellular fgr gene, we have chosen to clone cDNA molecules representing the c-fgr transcript. Thus, cDNA was synthesized from peripheral blood mononuclear cell RNA and cloned into the Okayama-Berg expression vector. Utilizing v-fgr DNA fragments as probes, we identified seven cDNA clones. All of these clones were shown to represent human fgr gene transcripts by Southern analysis of genomic DNA as well as northern analysis of peripheral blood mononuclear cell RNA. The DNA sequence of one cDNA clone, 2.3 kb in length, showed 95% homology at the nucleotide level with that of v-fgr.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Epidemiology And Genetics (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CP005467-01
Application #
3963554
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Cancer Epidemiology and Genetics
Department
Type
DUNS #
City
State
Country
United States
Zip Code