Abstract

cDNA array is a powerful method that allows for rapid analysis of many genes differentially expressed in a variety of tissues of interest. Using Affymetrix oligo-nucleotide based cDNA arrays, we have so far surveyed 65 primary tissues and examined gene expression differences between the tumor and normal lung tissues. Among the molecular pathways affected in lung cancers, we observed a consistent down regulation of genes involved in the TGF-beta signaling. We further observed that the loss of SMAD6 and SMAD7 appeared to be correlated with an improved tumor-related survival in NSCLC patients. In vitro experiments using siRNA technology are currently underway to examine the mechanism of SMAD6 inactivation in lung tumors. We are also using well established assays of TGF-beta functions to examine their functional differences between the lung tumor and normal cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Epidemiology And Genetics (NCI)
Type
Intramural Research (Z01)
Project #
1Z01CP010162-03
Application #
6954039
Study Section
(LPG)
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2003
Total Cost
Indirect Cost

  Institution

Name
Cancer Epidemiology and Genetics
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Zhang, Jinghui; Finney, Richard P; Rowe, William et al. (2007) Systematic analysis of genetic alterations in tumors using Cancer Genome WorkBench (CGWB). Genome Res 17:1111-7
Hong, Kyeong-Man; Yang, Sei-Hoon; Chowdhuri, Sinchita Roy et al. (2007) Inactivation of LLC1 gene in nonsmall cell lung cancer. Int J Cancer 120:2353-8
Dracheva, Tatiana; Philip, Reena; Xiao, Wenming et al. (2007) Distinguishing lung tumours from normal lung based on a small set of genes. Lung Cancer 55:157-64
Tsurutani, Junji; Fukuoka, Junya; Tsurutani, Hiroko et al. (2006) Evaluation of two phosphorylation sites improves the prognostic significance of Akt activation in non-small-cell lung cancer tumors. J Clin Oncol 24:306-14
Yang, Sei Hoon; Mechanic, Leah E; Yang, Ping et al. (2005) Mutations in the tyrosine kinase domain of the epidermal growth factor receptor in non-small cell lung cancer. Clin Cancer Res 11:2106-10
He, Ping; Varticovski, Lyuba; Bowman, Elise D et al. (2004) Identification of carboxypeptidase E and gamma-glutamyl hydrolase as biomarkers for pulmonary neuroendocrine tumors by cDNA microarray. Hum Pathol 35:1196-209
Krop, Ian; Player, Audrey; Tablante, Ana et al. (2004) Frequent HIN-1 promoter methylation and lack of expression in multiple human tumor types. Mol Cancer Res 2:489-94
Dracheva, Tatiana; Jen, Jin (2003) Profiling lung adenocarcinoma: insights from genes and more genes. Cancer Biol Ther 2:299-300