Over the past year, we have used in situ hybridization method to assess the relative expression of candidate genes selected by SAGE and cDNA array analysis. We used fresh frozen tissue sections from lung cancers with surrounding normal and hybridize the tissue with biotin labeled probes of candidate genes. The hybridization signal is then visuallized using a tyramide based method. We have thus far surveyed nearly 50 fresh frozen lung tumor samples and 21 of those were suitable for in situ hybridization. Using these tissues, we have screened five genes for their differential expression in lung cancers and identified a potential tumor marker that is expecifically expressed in 90% of the tumors examed but not present in the normal lung. We are currently determining the relative expression of this candidate marker in a large panel of paraffin embedded lung tissues to determine the clinical significance of this gene's expression in lung cancer. We are also screening additional genes that are differentially expressed in lung cancer.
| Gill, Rajbir K; Vazquez, Madeline F; Kramer, Arin et al. (2008) The use of genetic markers to identify lung cancer in fine needle aspiration samples. Clin Cancer Res 14:7481-7 |
| Landi, Maria Teresa; Dracheva, Tatiana; Rotunno, Melissa et al. (2008) Gene expression signature of cigarette smoking and its role in lung adenocarcinoma development and survival. PLoS ONE 3:e1651 |
| Jin, Xiongjie; Zhang, Jianjun; Gao, Yanning et al. (2007) Relationship between mitochondrial DNA mutations and clinical characteristics in human lung cancer. Mitochondrion 7:347-53 |
| Zhang, Jinghui; Finney, Richard P; Rowe, William et al. (2007) Systematic analysis of genetic alterations in tumors using Cancer Genome WorkBench (CGWB). Genome Res 17:1111-7 |
| Hong, Kyeong-Man; Yang, Sei-Hoon; Chowdhuri, Sinchita Roy et al. (2007) Inactivation of LLC1 gene in nonsmall cell lung cancer. Int J Cancer 120:2353-8 |
| Dracheva, Tatiana; Philip, Reena; Xiao, Wenming et al. (2007) Distinguishing lung tumours from normal lung based on a small set of genes. Lung Cancer 55:157-64 |
| Hong, Kyeong-Man; Yang, Sei-Hoon; Guo, Mingzhou et al. (2005) Semiautomatic detection of DNA methylation at CpG islands. Biotechniques 38:354, 356, 358 |
| Yang, Sei Hoon; Mechanic, Leah E; Yang, Ping et al. (2005) Mutations in the tyrosine kinase domain of the epidermal growth factor receptor in non-small cell lung cancer. Clin Cancer Res 11:2106-10 |
| Dracheva, Tatiana; Jen, Jin (2003) Profiling lung adenocarcinoma: insights from genes and more genes. Cancer Biol Ther 2:299-300 |
