The goal of this project is to identify the target genes regulated by PAX3. Information obtained from studying PAX3 expression in a mouse model (Splotch) will be used to identify candidate PAX3 target genes. Genes regulated by PAX3 are being identified by WGPCR. Some of these genes are expected to be targets of PAX3 and expressed in the inner ear. Other targets of PAX3 that we identify may be important in understanding alveolar rhabdomyocarcoma since this form of childhood cancer results from a fusion of the DNA binding domain of PAX3 and the carbox-terminus transcription activation domain of the forked-head gene due to a causative translocation event in alveolar rhabdomyocarcoma cancer cells.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Intramural Research (Z01)
Project #
1Z01DC000040-04
Application #
6431982
Study Section
(LMG)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2000
Total Cost
Indirect Cost
Name
Deafness & Other Communication Disorders
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Barber, T D; Barber, M C; Cloutier, T E et al. (1999) PAX3 gene structure, alternative splicing and evolution. Gene 237:311-9