Regulation of Sugar Transport and Metabolism in Lactic Acid Bacteria

Thompson, J.

Abstract

1. Amino acid-dependent sugar transport systems have been identified in various species of Fusobacteria. 2. The phosphoenolpyruvate (PEP): dependent sucrose phosphotransferase system has been found in F. mortiferum. The first purification of the ATP-dependent fructokinase from this organism has been achieved, and the N-terminal has been determined. 3. Sucrose 6-phosphate hydrolase has been purified to homogeneity from Lactococcus lactis, and the structural gene (scrA) has been localized on the chromosomally-encoded transposon, Tn5306. 4. Fructokinase from L.lactis has also been purified to homogeneity, and the scrK gene is also localized on transposon Tn5306, close to scrA. 5. Transposon Tn5306 is the first """"""""metabolic"""""""" transposon to be described in lactic acid bacteria. This large (~50-100kb?) fragment of 'mobile' DNA encodes genes for N5-(CE)ornithine synthase, sucrose transport and catabolism, nisin synthesis and resistance, and a transposase gene.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Intramural Research (Z01)
• Project #: 1Z01DE000341-11
• Application #: 3854195
• Support Year: 11
• Fiscal Year: 1991

Institution

• Name: National Institute of Dental & Craniofacial Research
• Country: United States

Publications

Zhang, Qiangmin; Gao, Feng; Peng, Hao et al. (2009) Crystal structures of Streptococcus suis mannonate dehydratase (ManD) and its complex with substrate: genetic and biochemical evidence for a catalytic mechanism. J Bacteriol 191:5832-7

Zhang, Qiangmin; Peng, Hao; Gao, Feng et al. (2009) Structural insight into the catalytic mechanism of gluconate 5-dehydrogenase from Streptococcus suis: Crystal structures of the substrate-free and quaternary complex enzymes. Protein Sci 18:294-303

Hall, Barry G; Pikis, Andreas; Thompson, John (2009) Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations. Mol Biol Evol 26:2487-97

Pikis, Andreas; Hess, Sonja; Arnold, Ingrid et al. (2006) Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose. J Biol Chem 281:17900-8

Thompson, John; Hess, Sonja; Pikis, Andreas (2004) Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s). J Biol Chem 279:1553-61

Rajan, Shyamala S; Yang, Xiaojing; Collart, Frank et al. (2004) Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure 12:1619-29

Yip, Vivian L Y; Varrot, Annabelle; Davies, Gideon J et al. (2004) An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. J Am Chem Soc 126:8354-5

Xu, De-Qi; Thompson, John; Cisar, John O (2003) Genetic loci for coaggregation receptor polysaccharide biosynthesis in Streptococcus gordonii 38. J Bacteriol 185:5419-30

Cisar, J O; Xu, D Q; Thompson, J et al. (2000) An alternative interpretation of nanobacteria-induced biomineralization. Proc Natl Acad Sci U S A 97:11511-5