of Work: Mutational spectra provide information to help understand mechanisms underlying mutagenesis and carcinogenesis. We will examine forestomach tumors induced by a class of carcinogens (chloroprene, isoprene, and 1,3-butadiene) for activating mutations of the ras gene. This information will then be related to the known mutagenic properties of exogenously andendogenously-induced DNA adducts to provide information about the relevance of different adducts. Since these carcinogens induced tumors at multiple sites, mutational spectra for the other sites will be compared to that for the forestomach to determine whether a similar mechanism is involved. - Neither Human Subjects nor Human Tissues
| Sills, R C; Hong, H L; Boorman, G A et al. (2001) Point mutations of K-ras and H-ras genes in forestomach neoplasms from control B6C3F1 mice and following exposure to 1,3-butadiene, isoprene or chloroprene for up to 2-years. Chem Biol Interact 135-136:373-86 |
| Hayashi, S; Hong, H H; Toyoda, K et al. (2001) High frequency of ras mutations in forestomach and lung tumors of B6C3F1 mice exposed to 1-amino-2,4-dibromoanthraquinone for 2 years. Toxicol Pathol 29:422-9 |
