Abstract

First, we established the culture method for the embryos as early as day 5.5 embryos. We found that DMEM supplemented with 75% rat serum worked better than 100 % rat serum for E5.5 embryos whereas 100 % rat serum works better for E5.75 embryos. We are underway to optimize the condition to achieve the Cre-dependent gene disruption by Cre plasmids, since infection of recombinant Adenovirus for Cre was not sufficient enough to delete BMP receptors during cultivation. The knowledge that will be acquired through this research may give us better understanding for the primitive and definitive hematopoiesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES071004-09
Application #
7593957
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2007
Total Cost
$485,097
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Miura, Shigeto; Mishina, Yuji (2007) The DVE changes distal epiblast fate from definitive endoderm to neurectoderm by antagonizing nodal signaling. Dev Dyn 236:1602-10
Di-Gregorio, Aida; Sancho, Margarida; Stuckey, Daniel W et al. (2007) BMP signalling inhibits premature neural differentiation in the mouse embryo. Development 134:3359-69
Song, Lanying; Fassler, Reinhard; Mishina, Yuji et al. (2007) Essential functions of Alk3 during AV cushion morphogenesis in mouse embryonic hearts. Dev Biol 301:276-86
Miura, Shigeto; Davis, Shannon; Klingensmith, John et al. (2006) BMP signaling in the epiblast is required for proper recruitment of the prospective paraxial mesoderm and development of the somites. Development 133:3767-75
Park, Changwon; Lavine, Kory; Mishina, Yuji et al. (2006) Bone morphogenetic protein receptor 1A signaling is dispensable for hematopoietic development but essential for vessel and atrioventricular endocardial cushion formation. Development 133:3473-84
Kishigami, Satoshi; Komatsu, Yoshihiro; Takeda, Haruko et al. (2006) Optimized beta-galactosidase staining method for simultaneous detection of endogenous gene expression in early mouse embryos. Genesis 44:57-65
Fukuda, Tomokazu; Scott, Gregory; Komatsu, Yoshihiro et al. (2006) Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2. Genesis 44:159-67
Kishigami, Satoshi; Mishina, Yuji (2005) BMP signaling and early embryonic patterning. Cytokine Growth Factor Rev 16:265-78
Gaussin, Vinciane; Morley, Gregory E; Cox, Luk et al. (2005) Alk3/Bmpr1a receptor is required for development of the atrioventricular canal into valves and annulus fibrosus. Circ Res 97:219-26
Oh, Hidemasa; Chi, Xuan; Bradfute, Steven B et al. (2004) Cardiac muscle plasticity in adult and embryo by heart-derived progenitor cells. Ann N Y Acad Sci 1015:182-9

Showing the most recent 10 out of 16 publications