Abstract

A mass spectrometry-based Protein Microcharacterization Facility has been established within the Mass Spectrometry Workgroup in LSB. The purpose of the Facility is to provide state-of-the-art mass spectrometric characterization of proteins. This includes proteins separated by gel electrophoresis, obtained from expression systems, isolated from immunoprecipitation experiments, and identification of posttranslational modifications and adducts. The Facility also provides MS-based proteomics identifications in collaboration with the 2-D gel electrophoresis group of Alex Merrick in the NCT. Proteomics experiment proposals are reviewed by a screening committee prior to acceptance.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES101564-02
Application #
7007527
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2004
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Solanki, Sumeet; Dube, Prabhatchandra R; Birnbaumer, Lutz et al. (2017) Reduced Necrosis and Content of Apoptotic M1 Macrophages in Advanced Atherosclerotic Plaques of Mice With Macrophage-Specific Loss of Trpc3. Sci Rep 7:42526
Cuthbertson, Brandon J; Deterding, Leesa J; Williams, Jason G et al. (2008) Diversity in penaeidin antimicrobial peptide form and function. Dev Comp Immunol 32:167-81
Pi, Jingbo; Bai, Yushi; Reece, Jeffrey M et al. (2007) Molecular mechanism of human Nrf2 activation and degradation: role of sequential phosphorylation by protein kinase CK2. Free Radic Biol Med 42:1797-806
Choi, Jae H; Williams, Jason; Cho, Jaiesoon et al. (2007) Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress. J Biol Chem 282:30763-75
Lemieux, George A; Blumenkron, Fernando; Yeung, Nolan et al. (2007) The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10. J Biol Chem 282:14836-44
Vitorino, Rui; Calheiros-Lobo, Maria Joao; Williams, Jason et al. (2007) Peptidomic analysis of human acquired enamel pellicle. Biomed Chromatogr 21:1107-17
Vitorino, Rui; Ferreira, Rita; Neuparth, Maria et al. (2007) Subcellular proteomics of mice gastrocnemius and soleus muscles. Anal Biochem 366:156-69
Chu, Pei-Hsuan; Huang, Teng-Yi; Williams, Jason et al. (2006) Purified vitamin K epoxide reductase alone is sufficient for conversion of vitamin K epoxide to vitamin K and vitamin K to vitamin KH2. Proc Natl Acad Sci U S A 103:19308-13
Alves, Gelio; Yu, Yi-Kuo (2005) Robust accurate identification of peptides (RAId): deciphering MS2 data using a structured library search with de novo based statistics. Bioinformatics 21:3726-32
Vitorino, Rui; Lobo, Maria Joao C; Duarte, Jose et al. (2004) In vitro hydroxyapatite adsorbed salivary proteins. Biochem Biophys Res Commun 320:342-6

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