In recent years there has been increasing evidence that hormones are capable of regulating the immune system. It has been suggested that prolactin is an immunostimulatory hormone and that reduction of serum prolactin levels in experimental animals by hypophysectomy or treatment with bromocriptine will result in a degree of immunosuppression. An animal model of experimental autoimmune uveitis (EAU) induced by immunization of rats with S-antigen (a soluble antigen from the retina) is used to study intraocular inflammatory disease. We have demonstrated a decrease in antibody production in both male and female rats and a decreased incidence of uveitis in female animals. No significant effect on the immune responses, as measured by lymphocyte proliferation, was seen. As reported before, high doses of cyclosporine (10 mg/kg) result in only partial reduction of intraocular inflammation. We have demonstrated that the suppression of prolactin by concurrent use of bromocriptine in combination with low-dose cyclosporine is more effective than either drug separately in suppressing both the incidence of disease and cellular and humoral immune responses. Evidence in the literature suggests that cyclosporine competes with prolactin for binding sites on lymphocytes. Reductions in prolactin level may reduce competition for those sites and make cyclosporine treatment more effective. Further studies with this animal model will elucidate other aspects of the neuroendocrine axis that may be used to regulate the immune system to treat autoimmune diseases.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000220-04
Application #
3898141
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost
Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code