Insertional mutagenesis and ear development

Burgess, Shawn M.

Abstract

The main emphasis of the section is to use zebrafish as a model organism and molecular genetics as the technique to identify the function of new genes important in human development. The section focuses on isolating embryonic mutations which affect the normal development of the zebrafish ear as well as interfere with its function. Some of these genes will possibly have importance for congenital deafness in humans. The section also is developing techniques for using retroviruses as a tool for large scale analysis of gene function on a genome-wide scale. Current projects involve analyizing 4 mutations that affect early zebrafish ear development. The genes will be cloned and expression analyzed. Additonally, new retroviral vectors are being developed that will allow for systematic screening of gene expression in vivo.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Human Genome Research Institute (NHGRI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HG000183-01
• Application #: 6555938
• Study Section: (GTB)
• Support Year: 1
• Fiscal Year: 2001

Institution

• Name: Human Genome Research
• Country: United States

Publications

Gomez, Gustavo A; Veldman, Matthew B; Zhao, Yan et al. (2009) Discovery and characterization of novel vascular and hematopoietic genes downstream of etsrp in zebrafish. PLoS One 4:e4994

Jao, Li-En; Burgess, Shawn M (2009) Production of pseudotyped retrovirus and the generation of proviral transgenic zebrafish. Methods Mol Biol 546:13-30

Liang, Jin; Burgess, Shawn M (2009) Gross and fine dissection of inner ear sensory epithelia in adult zebrafish (Danio rerio). J Vis Exp :

Wang, Ting; Zeng, Jue; Lowe, Craig B et al. (2007) Species-specific endogenous retroviruses shape the transcriptional network of the human tumor suppressor protein p53. Proc Natl Acad Sci U S A 104:18613-8

Wang, Dongmei; Jao, Li-En; Zheng, Naizhong et al. (2007) Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions. Proc Natl Acad Sci U S A 104:12428-33

Wu, Xiaolin; Luke, Brian T; Burgess, Shawn M (2006) Redefining the common insertion site. Virology 344:292-5

Antonellis, Anthony; Lee-Lin, Shih-Queen; Wasterlain, Amy et al. (2006) Functional analyses of glycyl-tRNA synthetase mutations suggest a key role for tRNA-charging enzymes in peripheral axons. J Neurosci 26:10397-406

Ahmed, Zubair M; Goodyear, Richard; Riazuddin, Saima et al. (2006) The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. J Neurosci 26:7022-34

Yan, Jizhou; Xu, Lisha; Crawford, Gregory et al. (2006) The forkhead transcription factor FoxI1 remains bound to condensed mitotic chromosomes and stably remodels chromatin structure. Mol Cell Biol 26:155-68

Nakai, Hiroyuki; Wu, Xiaolin; Fuess, Sally et al. (2005) Large-scale molecular characterization of adeno-associated virus vector integration in mouse liver. J Virol 79:3606-14