Six novel murine homeobox genes, Uncx-4.1, OG-2, OG-9, OG-12, OG-22, and NKx were cloned and partially sequenced. Two OG-12 cDNAs were cloned that correspond to alternatively spliced species of OG-12 mRNA. More than ten kb of NKx genomic DNA were sequenced. Northern blot hybridization of NKx poly-A+ RNA from adult mouse tissues revealed one major band of NKx RNA in brain and multiple bands in testes. Nkx mRNA was found in the mesencephalon, myelencephalon, spinal cord, vertebrae, and ribs of 12 day mouse embryos, and in distinct patches in the pons and diencephalon of 14 day mouse embryos. Two species of OG-22 cDNA were found in mouse embryo cDNA libraries that correspond to alternatively spliced species of OG-22 mRNA. OG-22 mRNA is expressed in the head and brachial arches of mouse embryos, and in adult mouse lung and testis.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL000157-04
Application #
6162630
Study Section
Special Emphasis Panel (LBG)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1997
Total Cost
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Cinquanta, M; Rovescalli, A C; Kozak, C A et al. (2000) Mouse Sebox homeobox gene expression in skin, brain, oocytes, and two-cell embryos. Proc Natl Acad Sci U S A 97:8904-9
Rovescalli, A C; Cinquanta, M; Ferrante, J et al. (2000) The mouse Nkx-1.2 homeobox gene: alternative RNA splicing at canonical and noncanonical splice sites. Proc Natl Acad Sci U S A 97:1982-7