The cytotoxicity associated with the lipid peroxidation is due in part to the conjugation of amino acid residues in protein with 4-hydroxy-2- nonenal which is a product of lipid peroxidation. A highly sensitive immunochemical method was developed for the detection and quantitation of 4-hydroxynonenal (HNE)-protein conjugates. With this method, it was demonstrated that HNE-protein conjugates are produced when crude homogenates of rat liver are treated with HNE or are subjected to oxidative stresses obtained by exposure to tert-butylhydroperoxide or to metal-ion-catalyzed oxidation systems.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL000276-03
Application #
3779484
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code