Abstract

Mono-ADP-ribosylation is a reversible modification of proteins, with NAD: arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases catalyzing opposing arms of an ADP-ribosylation cycle. Prior studies have examined the structure of the mouse ADP-ribosylarginine hydrolase gene. The 5 flanking promoter region was shown to have a critical regulatory element at -199 to -89. This region was found to function in a number of cell lines, suggesting the requirement for a relatively common transcription factor. Further mutational analysis of the promoter and electrophoretic mobility shift assays identified a positive GC-box element (-107- -95); using supershift assays, Sp1 and Sp3 were found to bind to this motif. In cotransferction experiments using Drosophila SL2 cells, which lack endogenous Sp1, Sp1 was found to transactivate the ADP-ribosylarginine hydrolase promoter in a manner dependent on the presence of an intact Sp1-binding motif. The promoter activity pattern and involvement of Sp1 transcription factors are consistent with prior observations showing widespread hydrolase expression in mammalian tissues. - ADP-ribosylation, ADP- ribosylarginine hydrolase

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL000659-07
Application #
6290384
Study Section
Special Emphasis Panel (PCCM)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Hisatsune, Junzo; Nakayama, Masaaki; Isomoto, Hajime et al. (2008) Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation. J Immunol 180:5017-27
Kim, Richard D; Greenberg, David E; Ehrmantraut, Mary E et al. (2008) Pulmonary Nontuberculous Mycobacterial Disease: Prospective Study of a Distinct Preexisting Syndrome. Am J Respir Crit Care Med :
Morinaga, Naoko; Yahiro, Kinnosuke; Matsuura, Gen et al. (2008) Subtilase cytotoxin, produced by Shiga-toxigenic Escherichia coli, transiently inhibits protein synthesis of Vero cells via degradation of BiP and induces cell cycle arrest at G1 by downregulation of cyclin D1. Cell Microbiol 10:921-9
Steagall, Wendy K; Barrow, Bethany J; Glasgow, Connie G et al. (2007) Beta-2-adrenergic receptor polymorphisms in cystic fibrosis. Pharmacogenet Genomics 17:425-30
Kato, Jiro; Zhu, Jianfeng; Liu, Chengyu et al. (2007) Enhanced sensitivity to cholera toxin in ADP-ribosylarginine hydrolase-deficient mice. Mol Cell Biol 27:5534-43
Morinaga, Naoko; Yahiro, Kinnosuke; Matsuura, Gen et al. (2007) Two distinct cytotoxic activities of subtilase cytotoxin produced by shiga-toxigenic Escherichia coli. Infect Immun 75:488-96
Harada, Naoki; Yasunaga, Ryoko; Higashimura, Yasuki et al. (2007) Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells. J Biol Chem 282:22651-61
Hisatsune, Junzo; Yamasaki, Eiki; Nakayama, Masaaki et al. (2007) Helicobacter pylori VacA enhances prostaglandin E2 production through induction of cyclooxygenase 2 expression via a p38 mitogen-activated protein kinase/activating transcription factor 2 cascade in AZ-521 cells. Infect Immun 75:4472-81
Ihara, Hideshi; Tsutsuki, Hiroyasu; Ida, Tomoaki et al. (2007) Alternative polyadenylation sites of human endothelial nitric oxide synthase mRNA. Biochem Biophys Res Commun 363:146-52
Morrison, Alan R; Moss, Joel; Stevens, Linda A et al. (2006) ART2, a T cell surface mono-ADP-ribosyltransferase, generates extracellular poly(ADP-ribose). J Biol Chem 281:33363-72

Showing the most recent 10 out of 47 publications