Methods of Ionization in Mass Spectroscopy

Markey, S P.

Abstract

The dual cell Fourier transform mass spectrometer has been tested in various modes of sample introduction and found to have several basic design flaws precluding its usage as a routine analytical instrument. Experiments using electron ionization - probe analysis, laser desorption, and gas chromatography have served to suggest alternative hardware and software designs which are presently being built and which will be installed in the next year. The instrument has been used to characterize synthetic intermediates and products, particularly in a high resolution mode. Collaborative studies on element and nuclide selective analyses using a microwave reaction interface to combust organic compounds and analyze the resulting species are continuing.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Mental Health (NIMH)
• Type: Intramural Research (Z01)
• Project #: 1Z01MH000274-12
• Application #: 3968416
• Support Year: 12
• Fiscal Year: 1986

Institution

• Name: U.S. National Institute of Mental Health
• Country: United States

Publications

Nanavati, Dhaval; Austin, Daniel R; Catapano, Lisa A et al. (2011) The effects of chronic treatment with mood stabilizers on the rat hippocampal post-synaptic density proteome. J Neurochem 119:617-29

Lockwich, Timothy; Pant, Jaya; Makusky, Anthony et al. (2008) Analysis of TRPC3-interacting proteins by tandem mass spectrometry. J Proteome Res 7:979-89

Turck, Christoph W; Falick, Arnold M; Kowalak, Jeffrey A et al. (2007) The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 study: relative protein quantitation. Mol Cell Proteomics 6:1291-8

Tuo, Jingsheng; Bojanowski, Christine M; Zhou, Min et al. (2007) Murine ccl2/cx3cr1 deficiency results in retinal lesions mimicking human age-related macular degeneration. Invest Ophthalmol Vis Sci 48:3827-36

Arnott, David; Gawinowicz, Mary Ann; Kowalak, Jeffrey A et al. (2007) ABRF-PRG04: differentiation of protein isoforms. J Biomol Tech 18:124-34

Glasker, Sven; Vortmeyer, Alexander O; Lonser, Russell R et al. (2006) Proteomic analysis of hemangioblastoma cyst fluid. Cancer Biol Ther 5:549-53

Fujigaki, Hidetsugu; Saito, Kuniaki; Lin, Felix et al. (2006) Nitration and inactivation of IDO by peroxynitrite. J Immunol 176:372-9

Zhuang, Zhengping; Huang, Steve; Kowalak, Jeff A et al. (2006) From tissue phenotype to proteotype: sensitive protein identification in microdissected tumor tissue. Int J Oncol 28:103-10

Masuda, Junichi; Maynard, Dawn M; Nishimura, Masayuki et al. (2005) Fully automated micro- and nanoscale one- or two-dimensional high-performance liquid chromatography system for liquid chromatography-mass spectrometry compatible with non-volatile salts for ion exchange chromatography. J Chromatogr A 1063:57-69

Chiba-Falek, Ornit; Kowalak, Jeffrey A; Smulson, Mark E et al. (2005) Regulation of alpha-synuclein expression by poly (ADP ribose) polymerase-1 (PARP-1) binding to the NACP-Rep1 polymorphic site upstream of the SNCA gene. Am J Hum Genet 76:478-92