Abstract

A method has been developed for the quantitative determination of the rates of local glucose consumption in the discrete functional and structural components of the brain in conscious or anesthetized laboratory animals. The method is based on the use of (14C)deoxyglucose as a tracer for glucose flux through the hexokinase step. Local [14C]deoxyglucose-6-phosphate concentrations in the tissues of the CNS are measured by a quantitative autoradiographic method. In as much as the autoradiographs of the relative rates of local glucose consumption can be used directly for metabolic mapping of functionally linked structures in the CNS, the method is being used to study alterations in the energy metabolism of the discrete functional and structural components of the brain in a variety of physiological, pharmacological, and pathological states.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH000882-19
Application #
3968463
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
19
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Poremba, Amy; Saunders, Richard C; Crane, Alison M et al. (2003) Functional mapping of the primate auditory system. Science 299:568-72
Itoh, Yoshiaki; Esaki, Takanori; Shimoji, Kazuaki et al. (2003) Dichloroacetate effects on glucose and lactate oxidation by neurons and astroglia in vitro and on glucose utilization by brain in vivo. Proc Natl Acad Sci U S A 100:4879-84
Esaki, Takanori; Suzuki, Hideyo; Cook, Michelle et al. (2003) Functional activation of cerebral metabolism in mice with mutated thyroid hormone nuclear receptors. Endocrinology 144:4117-22
Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Effects of dopamine receptor blockade on cerebral blood flow response to somatosensory stimulation in the unanesthetized rat. J Pharmacol Exp Ther 303:497-502
Cohen, David M; Wei, Jingna; O'Brian Smith, E et al. (2002) A method for measuring cerebral glucose metabolism in vivo by 13C-NMR spectroscopy. Magn Reson Med 48:1063-7
Esaki, Takanori; Itoh, Yoshiaki; Shimoji, Kazuaki et al. (2002) Blockade of K(ATP) channels with glibenclamide does not alter functional activation of cerebral blood flow in the unanesthetized rat. Brain Res 948:56-63
Itoh, Y; Esaki, T; Cook, M et al. (2001) Local and global cerebral blood flow and glucose utilization in the alpha-galactosidase A knockout mouse model of Fabry disease. J Neurochem 79:1217-24
Turkheimer, F; Pettigrew, K; Sokoloff, L et al. (2000) Selection of an adaptive test statistic for use with multiple comparison analyses of neuroimaging data. Neuroimage 12:219-29
Sokoloff, L (2000) In vivo veritas: probing brain function through the use of quantitative in vivo biochemical techniques. Annu Rev Physiol 62:24-Jan
Gotoh, J; Itoh, Y; Kuang, T Y et al. (2000) Negligible glucose-6-phosphatase activity in cultured astroglia. J Neurochem 74:1400-8

Showing the most recent 10 out of 15 publications