The expression of the genes coding for cholecystokinin (CCK), enkephalin and tyrosine hydroxylase (TH) was studied pre- and post-natally in the rat brain by using the corresponding specific cDNA probes to quantify mRNA. In addition, specific radioimmunoassays were used to measure CCK-immunoreactive peptides (CCK-IR) and enkephalin immunoreactive peptides. For the study on developmental expression of the tyrosine hydroxylase gene, mRNA levels were measured comparatively in the substantia nigra and the locus coeruleus and the levels of noradrenaline, dopamine and their metabolites were measured at the same time.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH002311-01
Application #
3968608
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code