During the development of a primary culture of chromaffin cells from adrenals of adult rats, we observed that the 2 month old cultured chromaffin cells extended neurites spontaneously if culture medium was not changed for a week. The result seems to imply that trophic material may be produced and accumulated in the culture medium of the chromaffin cells. Further studies on this observation now suggest that neurite promoting activity exists in conditioned medium from the adrenal chromaffin cells in culture. This neurite promoting factor was found to be protein in nature because it was inactivated by heating at 100 degrees and trypsin digestion. It is likely that this trophic activity is not due to NGF, fibroblast growth factors, epidermal growth factor, or insulin like growth factor. Neuropeptides, VIP, NPY, head activator peptide and motilin were all found to exert no effect on neurite out-growth from the cultured chromaffin cells. The exact nature of the trophic material detected in this study awaits further characterization. The proposed course of this study is to further characterize the putative trophic material detected in the culture medium of rat adrenal chromaffin cells. The study will include development of a new technique to detect and isolate a small quantity of trophic material.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Intramural Research (Z01)
Project #
1Z01MH002505-01
Application #
3880993
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
United States
Zip Code