The interaction between adenosine and PGE2 receptors linked to adenylate cyclase was investigated in cultured endothelial cells derived from dissociated cerebral microvessels. PGE2 stimulated adenylate cyclase in dose dependent manner (1nm-100 mu M) while adenosine increased the activity of adenylate cyclase only at high concentration of the drug (0.01- 0.1mM). Increasing concentration of adenosine with PGE2 (10- 6M) had a statistically significant effect on adenylate cyclase stimulation only at lower doses of adenosine (1-10nM). The same concentrations of adenosine (1-10nM) alone did not enhance significantly the formation of cAMP. Those results indicate that both vasoactive substances adenosine and PGE2 have an effect on receptors linked to adenylate cyclase.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002275-12
Application #
3945218
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1987
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code