Gene targeting in embryonic stem (ES) cells is being used to inactivate (knock-out) genes and use the mutated ES cells to generate mice with a mutation at the targeted locus. We have targeted the pro-opioelanocortin (POMC) gene in embryonic stem cells in order to determine the influence of POMC during development and post-natally. The POMC-targeted ES cells are now being used to generate chimeric animals that may carry the gene defect in the germline. Similarly, the cystatin C gene has been cloned and gene targeting constructs have been made in order to inactivate the cystatin C gene in ES cells. Cystatin-C mice will be made and the mutated human cystatin C gene from a patient with hereditary cerebral angiopathy will be introduced into one of the constructs in order to make a mouse model for hereditary stroke.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002878-02
Application #
3760356
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1994
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code