Since it is known that the tat protein of the HIV will enter the cell in vitro and stimulate the production of HIV products, there is almost certainly a receptor site for it on the cell membrane. Segments of this 86 amino acid protein are being synthesized to determine which segment is responsible for binding to the receptor. This phenomenon will be evaluated by quantitatively measuring the binding force between the various segments and several types of human cells grown in tissue culture. We hope to use a flow cell and image analysis to determine the average shear force needed to detach the cells. The cells will then be attached to the bottom of a 96-well plate, which will be moved automatically from well to well under computer control.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Intramural Research (Z01)
Project #
1Z01RR010362-02
Application #
3852964
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost
Name
National Center for Research Resources
Department
Type
DUNS #
City
State
Country
United States
Zip Code