A fiber-optic-based instrument was developed to make simultaneous measurements of inter- and intracellular pH and membrane potential of vesicles containing bacteriorhodopsin in the presence of high-intensity actinic light. Fluorescent probes are incorporated into the membrane or intracellular space, and are excited at their characteristic wavelength. Most frequently, pyrinine and oxanol V or VI correlated independently with pH and ion-selective electrodes.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Intramural Research (Z01)
Project #
1Z01RR010465-01
Application #
5204134
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1995
Total Cost
Indirect Cost
Name
National Center for Research Resources
Department
Type
DUNS #
City
State
Country
United States
Zip Code