The post-mortem distributions and concentrations of calcium, silicon, and aluminum in plaque cores from brains of patients with Alzheimer's disease are being determined from elemental images and spectra acquired by wavelength-dispersive x-ray spectroscopy on a computer-controlled Cameca MBX electron beam x-ray microanalyzer. Both airdried and freeze-dried cryo- sections 8-10 micrometers thick from fresh frozen specimens with no chemical preparation are being analyzed. The drying methods will be compared for effect on elemental preservation and morphology, and quantitative results compared to those obtained by other workers using fixed material or purified cores.

Agency
National Institute of Health (NIH)
Institute
Division of Research Services (DRS)
Type
Intramural Research (Z01)
Project #
1Z01RS010254-01
Application #
3959974
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Research Services
Department
Type
DUNS #
City
State
Country
United States
Zip Code