Transmission electron microscopy (EM) is used as an adjunct procedure in the diagnosis of a wide range of neoplastic and non neoplastic diseases and can be extremely useful in well chosen diagnostic dilemmas. The main objectives of the diagnostic EM service are: (1) to provide EM diagnosis in patient specimens (2) to contribute to the understanding of the pathogenesis of diseases studied in various NIH protocols and (3) to assist investigators in research protocols with specific EM-related questions. The last year's accomplishments are summarized below:(1) A total of 122 patient tissues were processed and diagnosed by EM. Successful CAP inspection occurred on 2/15/00 leading to CAP certification (2) An additional 117 research specimens with multiple samples per specimen were processed and evaluated, resulting in 5 publications. (3) We explored the possibility to use a non radioisotopic in situ polymerase chain reaction at the ultrastrucutral level for the detection of oncogenes. An abstract submitted to the USCAP Academy of Pathology meeting in March of 1999 (Lab Invest 2000; 80:225A (1325)) won honorary mention from the Society of Ultrastructural Pathology (4) Immuno EM was introduced to assist NIH investigators in resolving questions with clinical relevance. Examples and puplications are shown below: (a) Immuno EM labeling showed the human immunodeficiency virus (HIV) envelope protein gp4 to accumulate as an extracellular aggregate in the brains of HIV-infected patients with dementia - Caffrey, M. et al: J Biol Chem 2000; 275:19877-19882 .(b) Immuno EM labeling showed that in patients susceptible to isoflurane-induced hepatitis, isufluorane metabolites bind to liver proteins and are localized in mitochondria- Njoku, D. et al: (Submitted).(c) Immuno EM labeling showed sites of leptin localization in cultured preadipocytes and mature tissue adipocytes- Bornstein, S.R. et al. Diabetes 2000; 49:532-538 .

Agency
National Institute of Health (NIH)
Institute
Division of Clinical Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC009394-07
Application #
6433406
Study Section
(LP)
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2000
Total Cost
Indirect Cost
Name
Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Martin-Manso, Gema; Galli, Susana; Ridnour, Lisa A et al. (2008) Thrombospondin 1 promotes tumor macrophage recruitment and enhances tumor cell cytotoxicity of differentiated U937 cells. Cancer Res 68:7090-9
Lodish, Maya B; Powell, Anathea C; Abu-Asab, Mones et al. (2008) Insulinoma and gastrinoma syndromes from a single intrapancreatic neuroendocrine tumor. J Clin Endocrinol Metab 93:1123-8
Lee, In Hye; Cao, Liu; Mostoslavsky, Raul et al. (2008) A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy. Proc Natl Acad Sci U S A 105:3374-9
Yang, Youfeng; Padilla-Nash, Hesed M; Vira, Manish A et al. (2008) The UOK 257 cell line: a novel model for studies of the human Birt-Hogg-Dube gene pathway. Cancer Genet Cytogenet 180:100-9
Valencia, Julio C; Rouzaud, Francois; Julien, Sylvain et al. (2007) Sialylated core 1 O-glycans influence the sorting of Pmel17/gp100 and determine its capacity to form fibrils. J Biol Chem 282:11266-80
Alesci, Salvatore; Perera, Shiromi M; Lai, Edwin W et al. (2007) Adenoviral gene transfer in bovine adrenomedullary and murine pheochromocytoma cells: potential clinical and therapeutic relevance. Endocrinology 148:3900-7
Isenberg, Jeff S; Hyodo, Fuminori; Matsumoto, Ken-Ichiro et al. (2007) Thrombospondin-1 limits ischemic tissue survival by inhibiting nitric oxide-mediated vascular smooth muscle relaxation. Blood 109:1945-52
Steel, Jason C; Cavanagh, Heather M A; Burton, Mark A et al. (2007) Increased tumor localization and reduced immune response to adenoviral vector formulated with the liposome DDAB/DOPE. Eur J Pharm Sci 30:398-405
Isenberg, Jeff S; Romeo, Martin J; Abu-Asab, Mones et al. (2007) Increasing survival of ischemic tissue by targeting CD47. Circ Res 100:712-20
Ge, Yun; Montano, Idalia; Rustici, Gabriella et al. (2006) Selective leukemic-cell killing by a novel functional class of thalidomide analogs. Blood 108:4126-35

Showing the most recent 10 out of 23 publications