Identifying genes and gene products that are important in tumor cells is a high priority goal of the National Cancer Institute. In order to determine whether a gene of interest is expressed in human malignancy, investigators must be able to evaluate actual tissue samples of human tumors. Unfortunately, access to large numbers of well-characterized human tissue samples is difficult, and there is a large expense for the preparation of glass slides from tumor in order to perform immunohistochemistry or in-situ hybridization. As a partial solution to this problem, pathologists began to combine multiple tissue samples in one tissue block, in order to reduce the histology costs and the time and effort involved to perform the special studies. This technique has been refined to the point where hundreds of tissue samples can be placed in a grid arrangement in a single paraffin tissue block. Investigators in the NHGRI (Kohenen, Kallioniemi and others) demonstrated the utility of large scale tissue microarrays in a seminal paper published in Science. Following a tissue microarray workshop hosted by NCI in Fall, 1999, a pathology-based tissue microarray core facility was established. This project is a direct outgrowth of the Extraordinary Opportunity to Define the Signatures of Cancer Cells identified in the 2001 Bypass Budget plan. The goals of the first year were to establish a core microarray production facility based on a similar facility in the NHGRI and to create the first mixed tumor microarrays for nationwide distribution. The initial product of the core was a mixed tumor block containing representative samples of the most common epithelial malignancies (breast, colon, lung, prostate, and ovary) as well as samples of melanoma, glioma and lymphoma. A selection of normal tissue and standard cell lines was also included to bring the total number of tissue spots to 500. Anonymized human tumor samples are obtained through the CHTN, and this organization will also handle distribution of arrays on glass slides to investigators in the intramural and extramural scientific community. Four sets of multi-tumor arrays were prepared in the first production year (FY2001), totaling more than 3000 slides for distribution. In the current phase of this project we are collaborating with mainly intramural investigators on tumor specific projects, both building arrays as well as staining and analyzing the pathology. This past year (FY2005) we have built arrays of the NCI60 cell lines and xenografts from those cell lines, pediatric tumors, breast carcinoma, non-small cell lung carcinoma, melanoma and general tumor arrays. In addition to slide production, the core facility has been engaged in technology development, from the basic histology procedures to array use to large-scale imaging of arrays. We have acquired an automated arrayer and have worked collaboratively on further technology development of the automated arrayer and an automated imaging system.
| Evans, Andrew; Bates, Victoria; Troy, Helen et al. (2008) Glut-1 as a therapeutic target: increased chemoresistance and HIF-1-independent link with cell turnover is revealed through COMPARE analysis and metabolomic studies. Cancer Chemother Pharmacol 61:377-93 |
| Bruce, Benjamin; Khanna, Gaurav; Ren, Ling et al. (2007) Expression of the cytoskeleton linker protein ezrin in human cancers. Clin Exp Metastasis 24:69-78 |
| Molinolo, Alfredo A; Hewitt, Stephen M; Amornphimoltham, Panomwat et al. (2007) Dissecting the Akt/mammalian target of rapamycin signaling network: emerging results from the head and neck cancer tissue array initiative. Clin Cancer Res 13:4964-73 |
| Goldstein, Neal S; Hewitt, Stephen M; Taylor, Clive R et al. (2007) Recommendations for improved standardization of immunohistochemistry. Appl Immunohistochem Mol Morphol 15:124-33 |
| Yang, Sherry X; Hewitt, Stephen M; Steinberg, Seth M et al. (2007) Expression levels of eIF4E, VEGF, and cyclin D1, and correlation of eIF4E with VEGF and cyclin D1 in multi-tumor tissue microarray. Oncol Rep 17:281-7 |
| Fukuoka, Junya; Dracheva, Tatiana; Shih, Joanna H et al. (2007) Desmoglein 3 as a prognostic factor in lung cancer. Hum Pathol 38:276-83 |
| Gannot, Gallya; Tangrea, Michael A; Erickson, Heidi S et al. (2007) Layered peptide array for multiplex immunohistochemistry. J Mol Diagn 9:297-304 |
| Takikita, Mikiko; Chung, Joon-Yong; Hewitt, Stephen M (2007) Tissue microarrays enabling high-throughput molecular pathology. Curr Opin Biotechnol 18:318-25 |
| Chung, Joon-Yong; Braunschweig, Till; Tuttle, Kimberly et al. (2007) Tissue microarrays as a platform for proteomic investigation. J Mol Histol 38:123-8 |
| Yang, Xiaohong R; Sherman, Mark E; Rimm, David L et al. (2007) Differences in risk factors for breast cancer molecular subtypes in a population-based study. Cancer Epidemiol Biomarkers Prev 16:439-43 |
Showing the most recent 10 out of 36 publications
