Abstract

One of the most significant accomplishments was to show a strong inhibitory properties of human defensin 5 (HD5) against the papillomaviruses. These properties make HD5 a potential candidate for the topical agent in preventing the papillomavirus infection.The other accomplishment was an identification of several structural determinants of chemotaxis in molecules of human beta-defensins. In broad structural and functional studies of mutants of human beta-defensin 1, we characterized (for the first time in case of defensins) a draft of the epitope involved in the binding and activation of the chemokine receptor CCR6.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Clinical Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC010386-05
Application #
7331715
Study Section
Mammalian Cell Lines Committee (MCL)
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2006
Total Cost
Indirect Cost

  Institution

Name
Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Wu, Zhibin; Li, Xiangqun; Ericksen, Bryan et al. (2007) Impact of pro segments on the folding and function of human neutrophil alpha-defensins. J Mol Biol 368:537-49
Zou, Guozhang; de Leeuw, Erik; Li, Chong et al. (2007) Toward understanding the cationicity of defensins. Arg and Lys versus their noncoded analogs. J Biol Chem 282:19653-65
Pazgier, M; Hoover, D M; Yang, D et al. (2006) Human beta-defensins. Cell Mol Life Sci 63:1294-313
Buck, Christopher B; Day, Patricia M; Thompson, Cynthia D et al. (2006) Human alpha-defensins block papillomavirus infection. Proc Natl Acad Sci U S A 103:1516-21
Pazgier, Marzena; Lubkowski, Jacek (2006) Expression and purification of recombinant human alpha-defensins in Escherichia coli. Protein Expr Purif 49:1-8
Xie, Cao; Prahl, Adam; Ericksen, Bryan et al. (2005) Reconstruction of the conserved beta-bulge in mammalian defensins using D-amino acids. J Biol Chem 280:32921-9
Economopoulou, Matina; Bdeir, Khalil; Cines, Douglas B et al. (2005) Inhibition of pathologic retinal neovascularization by alpha-defensins. Blood 106:3831-8
Wu, Zhibin; Cocchi, Fiorenza; Gentles, David et al. (2005) Human neutrophil alpha-defensin 4 inhibits HIV-1 infection in vitro. FEBS Lett 579:162-6
Yang, De; Biragyn, Arya; Hoover, David M et al. (2004) Multiple roles of antimicrobial defensins, cathelicidins, and eosinophil-derived neurotoxin in host defense. Annu Rev Immunol 22:181-215
Wu, Zhibin; Alexandratos, Jerry; Ericksen, Bryan et al. (2004) Total chemical synthesis of N-myristoylated HIV-1 matrix protein p17: structural and mechanistic implications of p17 myristoylation. Proc Natl Acad Sci U S A 101:11587-92

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