Purification of a novel remodeling enzyme

Pazin, Michael J.

Abstract

Previously, we identified a multi-subunit complex containing CHD5, and identified components of the complex. This complex is high molecular weight, as determined by gel filtration. We found CHD5 mRNA and protein specifically expressed in brain, in rat, mouse, and human. Progress: We have identified potential targets of CHD5 using overexpression in a cell line. Future: We will determine the binding sites for this remodeling enzyme, the mechanism of remodeling for this enzyme, and ultimately, the biological role for this enzyme.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Aging (NIA)
• Type: Investigator-Initiated Intramural Research Projects (ZIA)
• Project #: 1ZIAAG000378-02
• Application #: 7963952
• Support Year: 2
• Fiscal Year: 2009
• Total Cost: $266,756

Institution

• Name: National Institute on Aging

Publications

Gleichmann, Marc; Zhang, Yongqing; Wood 3rd, William H et al. (2012) Molecular changes in brain aging and Alzheimer's disease are mirrored in experimentally silenced cortical neuron networks. Neurobiol Aging 33:205.e1-18

Potts, Rebecca Casaday; Zhang, Peisu; Wurster, Andrea L et al. (2011) CHD5, a brain-specific paralog of Mi2 chromatin remodeling enzymes, regulates expression of neuronal genes. PLoS One 6:e24515

Zhang, Peisu; Casaday-Potts, Rebecca; Precht, Patricia et al. (2011) Nontelomeric splice variant of telomere repeat-binding factor 2 maintains neuronal traits by sequestering repressor element 1-silencing transcription factor. Proc Natl Acad Sci U S A 108:16434-9

Song, Shaoming; Kole, Sutapa; Precht, Patricia et al. (2010) Activation of heat shock factor 1 plays a role in pyrrolidine dithiocarbamate-mediated expression of the co-chaperone BAG3. Int J Biochem Cell Biol 42:1856-63

Xie, Yi; Kole, Sutapa; Precht, Patricia et al. (2009) S-glutathionylation impairs signal transducer and activator of transcription 3 activation and signaling. Endocrinology 150:1122-31

Zhang, Peisu; Pazin, Michael J; Schwartz, Catherine M et al. (2008) Nontelomeric TRF2-REST interaction modulates neuronal gene silencing and fate of tumor and stem cells. Curr Biol 18:1489-94