During FY14 we accomplished the following: 1. We initiated studies to examine the response of brain cells to pro-inflammatory cytokines. We started with neuronal cell lines and astrocytomas of human origin. Cells were treated with TNFα, IL-1βor RAGE-ligand for different time periods followed by RNA analysis. The kinetics of NF-κB p65 (RelA) translocation was determined by confocal microscopy, and dynamics of NF-κB binding to target sites is being determined by ChIP-Seq. Cumulatively, we expect that these studies will provide a comprehensive kinetic view of the inflammatory response of neurons and other brain-resident cell types. 2. To independently substantiate the observations in cell lines, we established a collaboration with Dr. Mark Mattsons section (LNS, NIA) to evaluate the responses of primary mouse neurons, microglia and astrocytes to pro-inflammatory cytokines. These studies are in progress.
| Munk, Rachel B; Sugiyama, Katsuki; Ghosh, Paritosh et al. (2011) Antigen-independent IFN-? production by human naïve CD4 T cells activated by IL-12 plus IL-18. PLoS One 6:e18553 |
| Olkhanud, Purevdorj B; Damdinsuren, Bazarragchaa; Bodogai, Monica et al. (2011) Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4? T cells to T-regulatory cells. Cancer Res 71:3505-15 |
| Sen, Ranjan (2011) The origins of NF-?B. Nat Immunol 12:686-8 |
