Abstract

The unit has completed backcrossing of sRAGEhigh mice to murine C57BL/6J genetic background with the aid of NCI congenic service. Partial phenotypic assessments and studies include the reaction of sRAGEhigh mice under septic shock, and sRAGEs influence on neutrophil extracellular trap-mediated intravascular cell death (NETosis), a process that has recently been found to be a prerequisite for atherogenesis. Additional work includes our units discovery of a subset of macrophages (small, MHCII positive and GATA-6 driven) that depend on RAGE for inflammatory reaction, polarity, and vascular transmigration. It is an ideal model to study the role of glycation in aging process and longevity. It is also the first in-house model generated in NIA for natural aging studies and can be used as a nutrition model if the mice are fed with AGE-enriched diets.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAG000875-08
Application #
9353046
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
Aging
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Low, Daren; Subramaniam, Renuka; Lin, Li et al. (2015) Chitinase 3-like 1 induces survival and proliferation of intestinal epithelial cells during chronic inflammation and colitis-associated cancer by regulating S100A9. Oncotarget 6:36535-50
Tae, Hyun-Jin; Petrashevskaya, Natalia; Ahmet, Ismayil et al. (2014) Vessel ultrasound sonographic assessment of soluble receptor for advanced glycation end products efficacy in a rat balloon injury model. Curr Ther Res Clin Exp 76:110-5
Wei, Wen; Kim, Ji Min; Medina, Danny et al. (2014) GeneOptimizer program-assisted cDNA reengineering enhances sRAGE autologous expression in Chinese hamster ovary cells. Protein Expr Purif 95:143-8
Tae, Hyun-Jin; Kim, Ji Min; Park, Sungha et al. (2013) The N-glycoform of sRAGE is the key determinant for its therapeutic efficacy to attenuate injury-elicited arterial inflammation and neointimal growth. J Mol Med (Berl) 91:1369-81
Pang, John; Zeng, Xiaokun; Xiao, Rui-ping et al. (2009) Design, generation, and testing of mammalian expression modules that tag membrane proteins. Protein Sci 18:1261-71
Lin, Li; Park, Sungha; Lakatta, Edward G (2009) RAGE signaling in inflammation and arterial aging. Front Biosci (Landmark Ed) 14:1403-13