Molecular Mechanisms Of Human Immunodeficiency Virus Trans-regulatory Proteins

Jeang, Kuan-Teh

Abstract

This project focuses on HIV-1 replication in cultured cells to develop understandings of viral pathogenesis and the cellular factors that influence viral gene expression. Important scientific advances that we have achieved in the 2009 to 2010 period include the following. 1) We have identified 252 cellular proteins that are important for HIV-1 replication in human T cells. 2) We have performed high throughput pyrosequencing of HIV-1 infected cells to identify changes in cellular small RNAs and a distribution of virus-produced small RNAs in cells. 3) We have characterized the role of an RNA-cap hypermethylating enzyme in regulating the expression of Rev-RRE-dependant HIV-1 unspliced/partially spliced RNAs.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Investigator-Initiated Intramural Research Projects (ZIA)
• Project #: 1ZIAAI000547-22
• Application #: 8156856
• Support Year: 22
• Fiscal Year: 2010
• Total Cost: $1,374,378

Institution

• Name: National Institute of Allergy and Infectious Diseases

Publications

Chen, Chia-Yen; Liu, Xiang; Boris-Lawrie, Kathleen et al. (2013) Cellular RNA helicases and HIV-1: insights from genome-wide, proteomic, and molecular studies. Virus Res 171:357-65

Zhang, Quan; Jeang, Kuan-Teh (2013) Long non-coding RNAs (lncRNAs) and viral infections. Biomed Pharmacother 3:34-42

Zhang, Quan; Chen, Chia-Yen; Yedavalli, Venkat S R K et al. (2013) NEAT1 long noncoding RNA and paraspeckle bodies modulate HIV-1 posttranscriptional expression. MBio 4:e00596-12

Tang, Sai-Wen; Ducroux, Aurelie; Jeang, Kuan-Teh et al. (2012) Impact of cellular autophagy on viruses: Insights from hepatitis B virus and human retroviruses. J Biomed Sci 19:92

Zheng, Yong-Hui; Jeang, Kuan-Teh; Tokunaga, Kenzo (2012) Host restriction factors in retroviral infection: promises in virus-host interaction. Retrovirology 9:112

Liu, Xiang; Houzet, Laurent; Jeang, Kuan-Teh (2012) Tombusvirus P19 RNA silencing suppressor (RSS) activity in mammalian cells correlates with charged amino acids that contribute to direct RNA-binding. Cell Biosci 2:41

Ammosova, Tatiana; Platonov, Maxim; Yedavalli, Venkat R K et al. (2012) Small molecules targeted to a non-catalytic ""RVxF"" binding site of protein phosphatase-1 inhibit HIV-1. PLoS One 7:e39481

Bachu, Mahesh; Yalla, Swarupa; Asokan, Mangaiarkarasi et al. (2012) Multiple NF-ýýB sites in HIV-1 subtype C long terminal repeat confer superior magnitude of transcription and thereby the enhanced viral predominance. J Biol Chem 287:44714-35

Klase, Zachary; Houzet, Laurent; Jeang, Kuan-Teh (2012) MicroRNAs and HIV-1: complex interactions. J Biol Chem 287:40884-90

Cunningham, Lea; Finckbeiner, Steven; Hyde, R Katherine et al. (2012) Identification of benzodiazepine Ro5-3335 as an inhibitor of CBF leukemia through quantitative high throughput screen against RUNX1-CBF? interaction. Proc Natl Acad Sci U S A 109:14592-7